Electrospun Nanofibers including Organic/Inorganic Nanohybrids: Polystyrene- and Clay-Based Architectures in Immunosensor Preparation for Serum Amyloid A

Diagnostic techniques based on biomolecules have application potential that can be realized in many fields, such as disease diagnosis, bioprocess imaging, food/beverage industries, and environmental pollutant imaging. Successful surface immobilization of is critical to increasing the stabilization, sensitivity, selectivity used bioassay systems. Nanofibers are good candidates for owing advantages morphology pore size. In this study, montmorillonite (MMT) clay modified with poly(amidoamine) (PAMAM) generation 3 (PAMAMG3) added polystyrene (PS) solutions, following which PS/MMT-PAMAMG3 nanofibers obtained using electrospinning method. The by testing PS% (wt%) MMT-PAMAMG3% ratios characterized scanning electron microscopy. Antiserum amyloid A antibody (Anti-SAA) then conjugated electrode via covalent bonds a zero-length cross linker. Finally, selective electrochemical determination serum (SAA) levels. linear range PS/MMT-PAMAM/Anti-SAA between 1 200 ng/mL SAA, detection limit 0.57 SAA. applicability PS/MMT-PAMAMG3/Anti-SAA investigated taking measurements synthetic saliva both containing